Hiring: Research Technician Company: Caltech Location: Pasadena, CA Job Posted Time: 2026-09-17 13:12:44 Employment Type: On-site About the job Experience: •2 years of hands-on experience with Sf9 and/or Hi5 cell culture and baculovirus-based protein expression. Required Skills: •Generate bacterial, insect cell, and mammalian expression constructs (basic molecular cloning techniques required). •Maintain and passage insect cells (Sf9 and Hi5) and mammalian cell cultures under sterile conditions. •Generate and amplify recombinant baculoviruses for protein expression in Sf9 and Hi5 cells. •Transfect and maintain various mammalian cell lines (e.g., HEK293, HTC116, Vero, HeLa) for transient and stable expression. •Optimize expression protocols for high-yield protein production in both systems. •Perform small- and large-scale protein expression experiments. •Harvest cells and assist with downstream, protein purification (modern chromatography methods). •Perform immunofluorescence microscopy to assess protein localization. Qualifications: •Bachelor’s degree in Biology, Biochemistry, Molecular Biology, or a related field. •Proficiency in aseptic technique and routine mammalian cell culture. •Operational familiarity with restriction enzyme cloning, western blotting, SDS-PAGE gel analysis, immunofluorescence microscopy, protein purification. •Strong organizational and communication skills. •Demonstrated capacity to work independently and collaboratively in a team environment. •Operational familiarity with serum-free media, virus titering, and MOI optimization. •Understanding of basic molecular cloning and transfection techniques. •Protein purification abilities are a plus but not necessary. •Prior experience in academic or industrial protein production environments. •The salary of the finalist(s) selected for this role will be set based on a variety of factors, including but not limited to, internal equity, experience, education, specialty and training. Compensation: •Competitive benefits and rewards package Interested candidates, please apply directly through the job posting on company's career page or try via AI auto apply on this platform. Don't miss this opportunity to join a forward-thinking team!